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Physiological and biochemical responses of Dosinia corrugata to different thermal and salinity stressors
Author(s) -
Nie Hongtao,
Zuo Shuqi,
Li Long,
Tian Cun,
Cao Chen,
Yan Xiwu
Publication year - 2018
Publication title -
journal of experimental zoology part a: ecological and integrative physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.834
H-Index - 11
eISSN - 2471-5646
pISSN - 2471-5638
DOI - 10.1002/jez.2152
Subject(s) - salinity , enzyme assay , estuary , temperature salinity diagrams , biology , chemistry , zoology , enzyme , ecology , biochemistry
Temperature and salinity are major factors affecting geographic distribution, reproduction, and physiological processes of marine and estuarine organisms. In this study, the effects of different temperatures and salinity on the respiratory metabolism and physiological response were evaluated in D. corrugata by measuring oxygen consumption rate (OCR), ammonia excretion rate (AER), and glycolytic enzyme activity. An increase in the OCR of D. corrugata with increasing temperature was observed. No peak of oxygen consumption was observed in D. corrugata over the temperature range evaluated. There was an increase in AERs with increasing temperature and salinity, respectively. With increasing salinity, the enzyme activity increased until it reached a peak at a salinity of 30 ppt, while the highest HK and LDH activity was observed at a salinity of 25 ppt. Our results may be used to optimize the temperature and salinity conditions for optimal growth and survival of D. corrugata and to provide basic information for conservation management and fishing moratorium of this economically important bivalve species. The enzyme activity decreased slightly from 15 to 20°C, but when the temperature exceeded 20°C, enzyme activity increased, reaching a maximum at 30°C (tested range of 10–30°C). With increasing salinity, the enzyme activity increased until it reached a peak at a salinity of 30 ppt, but once the salinity was greater than 30 ppt, the enzyme activity began to decrease up to salinity of 35 ppt.