Open AccessMicroscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model
Author(s) -
Linnemannstöns Karen,
Karuna M Pradhipa,
Witte Leonie,
Choezom Dolma,
HonemannCapito Mona,
Lagurin Alex Simon,
Schmidt Chantal Vanessa,
Shrikhande Shreya,
Steinmetz LaraKristin,
Wiebke Möbius,
Lenz Christof,
Gross Julia Christina
Publication year - 2022
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.1002/jev2.12263
Subject(s) - secretion , microbiology and biotechnology , endosome , rab , biogenesis , microvesicles , drosophila melanogaster , exocytosis , biology , extracellular vesicle , in vivo , multicellular organism , cell , biochemistry , gtpase , intracellular , microrna , genetics , gene
Extracellular vesicle (EV) secretion enables cell–cell communication in multicellular organisms. During development, EV secretion and the specific loading of signalling factors in EVs contributes to organ development and tissue differentiation. Here, we present an in vivo model to study EV secretion using the fat body and the haemolymph of the fruit fly, Drosophila melanogaster . The system makes use of tissue‐specific EV labelling and is amenable to genetic modification by RNAi. This allows the unique combination of microscopic visualisation of EVs in different organs and quantitative biochemical purification to study how EVs are generated within the cells and which factors regulate their secretion in vivo. Characterisation of the system revealed that secretion of EVs from the fat body is mainly regulated by Rab11 and Rab35, highlighting the importance of recycling Rab GTPase family members for EV secretion. We furthermore discovered a so far unknown function of Rab14 along with the kinesin Klp98A in EV biogenesis and secretion.