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Realization of noncontact confocal optical microsphere imaging microscope
Author(s) -
Zhou Yan,
Hong Minghui
Publication year - 2021
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23793
Subject(s) - confocal , microscope , microsphere , materials science , optics , confocal laser scanning microscope , optical microscope , confocal microscopy , confocal laser scanning microscopy , laser scanning , stereo microscope , biomedical engineering , contrast (vision) , laser , scanning electron microscope , physics , medicine , chemical engineering , engineering
A novel noncontact confocal optical microsphere imaging microscope (COMIM) is proposed to achieve high contrast optical nano‐imaging at a high scanning speed. The developed setup obtains images with significantly higher contrast when compared with conventional bright‐field microsphere nano‐imaging, as well as with a commercial confocal laser scanning microscope (CLSM). With the image‐by‐image scanning scheme, COMIM takes only 11% time to complete a three‐dimensional (3D) scanning image than using a commercial CLSM, while still offers an outstanding resolving power for the nano‐features on the sample.