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Stimulated emission depletion microscopy for biological imaging in four dimensions: A review
Author(s) -
Wu Zhaoyang,
Xu Xinzhu,
Xi Peng
Publication year - 2021
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23750
Subject(s) - sted microscopy , microscopy , stimulated emission , resolution (logic) , super resolution microscopy , nanotechnology , optics , materials science , physics , computer science , artificial intelligence , laser , scanning confocal electron microscopy
Stimulated emission depletion (STED) microscopy allows high lateral and axial resolution, long term imaging in living cells. Here we review recent technical advances in STED microscopy, with emphasis on resolution and measurement range of XYZt four dimensions. Different STED technical advances and novel STED probes are discussed with their respective application in biological subcellular imaging. This review may serve as a practical guide for choosing a suitable approach to the advanced STED super‐resolution imaging.