Premium
Golgi apparatus components in Entamoeba histolytica and Entamoeba dispar after monensin treatment
Author(s) -
TalamásLara Daniel,
AcostaVirgen Karla,
ChávezMunguía Bibiana,
LagunesGuillén Anel,
SalazarVillatoro Lizbeth,
EspinosaCantellano Martha,
MartínezPalomo Adolfo
Publication year - 2021
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23745
Subject(s) - golgi apparatus , entamoeba histolytica , immunoelectron microscopy , cytoplasm , biology , monensin , microbiology and biotechnology , endosome , vesicle , vacuole , endoplasmic reticulum , biochemistry , intracellular , membrane , immunohistochemistry , immunology
Highly dynamic ribosomes, glycogen granules, thinly fibrillar material, and multiple membrane‐bound vesicles are embedded in the matrix‐rich cytoplasm of Entamoeba spp. trophozoites. The absence of a Golgi apparatus in these amoebae has been commonly accepted. Here we challenge this observation by incubating Entamoeba histolytica and Entamoeba dispar with monensin, an ionophore that produces swelling of the Golgi apparatus. We observe changes in the trophozoites through standard transmission electron microscopy, cryofixation and cryosubstitution, and analyze the label and expression of known resident proteins of the cis‐GM130 and trans‐TGN38 Golgi network through confocal microscopy and Western blot assays. Cryosubstitution and standard methods using the treatment, preserved membranous lamellae resembling Golgi components. GM130 and TGN38 Golgi antigens were found by immunoelectron, immunoblot, and co‐localization by confocal microscopy using the reagent NBD C6‐ceramide. Our results indicate that previously undetected Golgi apparatus components are present in the cytoplasm of E. histolytica and E. dispar .