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Preparation and characterization of leukocyte‐ and platelet‐rich fibrin membrane derived from cats' blood
Author(s) -
Castilho Maíra Sales,
Rahal Sheila Canevese,
Dias Neto Ramiro das Neves,
Pereira Ana Cristina,
Francia Camila Contin Diniz de Almeida,
Mesquita Luciane dos Reis,
Antunes Carina Bueno,
Lainetti Patrícia de Faria,
FonsecaAlves Carlos Eduardo
Publication year - 2021
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23737
Subject(s) - buffy coat , platelet rich fibrin , fibrin , platelet , membrane , pathology , cats , chemistry , andrology , biology , biomedical engineering , microbiology and biotechnology , immunology , medicine , biochemistry
Autologous platelet concentrates have been used in regenerative medicine in humans due to the abundance of growth factors, but there are only a few reports in small animals. This study aimed to prepare and characterize a leukocyte and platelet‐rich fibrin membrane (L‐PRF) produced with blood obtained from cats. Thirteen client‐owned healthy adult Maine Coon cats were enrolled. The blood samples were collected and centrifuged at 650 g for 12 min using a centrifuge specifically designed for this application. The L‐PRF clot was removed from the tube and red blood cell base layer was separated, leaving buffy coat intact. After this, L‐PRF clot was compressed by specialized metal plate for 30–60 s, and L‐PRF membrane was obtained. Light microscopy examination of the membranes showed three distinct layers: white part, buffy coat, and red part. Immunohistochemical analysis demonstrated expression of vascular endothelial growth factor and platelet derived growth factor. The scanning electron microscopy showed that three‐dimensional architecture of fibrin network was more compact in the area near the buffy coat. In conclusion, the method used allowed the characterization of the L‐PRF membrane composition, which presented cell types and fibrin network architecture similar to those described in the human species.