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Antimicrobial, cytotoxic, antioxidants, enzyme inhibition activities, and scanning electron microscopy of Lactuca orientalis (Boiss.) Boiss. seeds
Author(s) -
Zahra Syeda Anber,
Iqbal Javed,
Abbasi Banzeer Ahsan,
Shahbaz Amir,
Kanwal Sobia,
Shah Syed Lal,
Ahmad Parvaiz,
Mahmood Tariq
Publication year - 2021
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23687
Subject(s) - phytochemical , dpph , traditional medicine , antimicrobial , brine shrimp , biology , antioxidant , flavonoid , agar diffusion test , chemistry , trichome , antibacterial activity , botany , biochemistry , microbiology and biotechnology , bacteria , medicine , genetics
Lactuca orientalis (Boiss.) Boiss. is one of the most frequently used ethnomedicinal plant. This research study was designed to decipher the phytochemical screening, pharmacological potential and implementation of scanning electron microscope (SEM). Six different solvents were used to prepare L. orientalis (LO) seed extracts. Phytochemical and antioxidant activities were determined calorimetrically. To investigate antidiabetic, α‐amylase inhibition assay was performed. Brine shrimp assay was performed for cytotoxicity and anti‐leishmanial via MTT assay. Disc‐diffusion assay was performed to detect protein kinase inhibitory, antibacterial and antifungal activities. SEM was used as identification tool. Significant amount of phenolic and flavonoid content were identified in methanol extract (LOSM) (95.76 ± 3.71 GAE/mg) and (77 ± 3.60 QE/mg). Highest DPPH scavenging potential (82%) was reported for LOSM. Significant total antioxidant capacity (90.60 ± 1.55 AAE/mg) and total reducing power (94.44 ± 1.38 AAE/mg) were determined for LOSM. Highest α‐amylase inhibition was found in LOSM (78.20 ± 1.58%). The highest LD 50 of brine shrimp was found for n‐Hexane extract (LOSH) 13.03 g/ml. All extracts showed strong anti‐leishmanial activity except LOSH. L. orientalis seeds showed significant protein kinase inhibition, antibacterial and antifungal activities. The seeds of L. orientalis were seen to be oblong to obovate, projections, wavy slightly straight, anticlinal wall was raised with apex acuminate. The outer‐periclinal wall convex with fine texture. In conclusion, our findings scientifically support ethnomedicinal and biological potentials of L. orientalis seeds. In future, L. orientalis seeds need to be explored for identification and isolation of bioactive compounds. The results obtained necessitate further in vivo studies to evaluate their pharmacological potentials.