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Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage
Author(s) -
Wang Guangshu,
Wang Zhenyu,
Sun Ning,
Cai Yurong,
Yang Xiaogang
Publication year - 2021
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23620
Subject(s) - sericin , horseradish peroxidase , hydrogen peroxide , reactive oxygen species , chemistry , silk , biophysics , intracellular , chemical engineering , biochemistry , materials science , enzyme , composite material , biology , engineering
Silk sericin microcapsules loaded with horseradish peroxidase (HRP) are prepared through protein self‐assembly in a green environment containing enzymes to protect liver cells from alcohol damage. Load content and release dynamics of HRP in sericin microcapsules are investigated. The role of HRP‐loaded microcapsules in hydrogen peroxide (H 2 O 2 ) degradation is demonstrated using electrochemical method. Furthermore, the effect of the HRP‐loaded microcapsules on cells and intracellular reactive oxygen species (ROS) level is evaluated using an alcohol damage model in vitro. Results show that HRP can be loaded effectively in the sericin microcapsules and can be released ROS‐responsively from microcapsules. Cell survival rate increases after suffering from alcohol damage due to the presence of HRP‐loaded microcapsule, and the active oxygen content in cells is maintained at a stable level even when it remained in an environment with high alcohol concentration. We believe that the internalized sericin microcapsules maintain HRP activity intracellularly, allow controlled HRP release within a host cell, and show excellent ability in antioxidative stress injury.