Premium
Effects of a biomimetic analog‐based experimental bonding system on caries‐affected and sound dentin
Author(s) -
Gomes Bianca Silva,
Rossi André Linhares,
Silva Eduardo Moreira,
Moreira Karla Tatiana Toro,
Santos Juliane Cucinello,
FerreiraPereira Antônio,
Portela Maristela Barbosa
Publication year - 2020
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23557
Subject(s) - dentin , adhesive , chemistry , nuclear chemistry , glutaraldehyde , materials science , phosphate , chromatography , composite material , biochemistry , organic chemistry , layer (electronics)
This study compared the ultrastructure, chemical composition, and proteases activity (PA) of sound (SD) and caries‐affected dentin (CAD) in the dentin hybrid layer after using an experimental bonding system containing pyromellitic dianhydride glycerol methacrylate and biomimetic analogs. The bonding system used a three step and a total‐etch procedure. Polyacrylic acid (5%) and sodium trimetaphosphate (5%) were added to the primer and monocalcium phosphate monohydrate (9%), beta‐tricalcium phosphate (10.5%), and calcium hydroxide (0.5%) were added to the adhesive. Transmission electron microscopy (TEM) was used to evaluate the resultant structure, particularly the adhesive‐dentin and the demineralized‐SD interfaces. The chemical composition was evaluated through energy‐dispersive X‐ray spectroscopy (EDS) and selected area electron diffraction (SAED). The PA was measured with the Coomassie Blue‐G250 coloring test, and the PA data were analyzed by ANOVA. EDS identified the presence of isolated calcium phosphate nanoparticles in the demineralized region; however, the SAED analysis did not show any evidences of hydroxyapatite (HA) neoformation in SD and CAD. The biomimetic analog‐based adhesive system inhibited the activities of dentin proteases immediately after treatment. Additionally, the proteolytic activity on the affected dentin resembled that of the SD. In conclusion, no HA formed in the demineralized SD and CAD although there were calcium and phosphate deposits. The experimental adhesive system inhibited dentin proteases.The present study uses a new approach to investigate the hybrid layer behavior in dentin. The experimental adhesive system was synthesized and used on sound and affected‐caries dentin as the substrate to reproduce real clinical conditions.