Premium
Multimodal imaging of skin lesions by using methylene blue as cancer biomarker
Author(s) -
Malik Sadia,
Andleeb Farah,
Ullah Hafeez
Publication year - 2020
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23555
Subject(s) - fluorescence , fluorescence lifetime imaging microscopy , skin cancer , optical imaging , pathology , histopathology , methylene blue , biomedical engineering , medicine , materials science , chemistry , cancer , optics , biochemistry , photocatalysis , catalysis , physics
This study aimed the optical imaging of malignant and normal skin tissues with multimodal wide‐field fluorescence polarization imaging (WF) technique, by using methylene blue as fluorescence dye. We present optical imaging of skin tissues by different techniques, including reflectance, fluorescence, and polarization imaging for early detection of skin cancer. We collected the reflectance confocal images at 390 and 500 nm. For wide‐field fluorescence images, specimens were stimulated at 640 nm and images were collected between 670 and 710 nm. The correlation of the regarded optical modalities with histopathology (H&E), their potentials, capabilities, and limitations to detect skin lesions are discussed. The advantages of multimodal imaging of skin tissues are analyzed to divulge possibilities for precise tumor boundary detection and their classification for malignant and nonmalignant skin tissues. Prior to imaging, the cells were stained in aqueous MB (a dye approved by FDA).