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Morphological analysis of aqueous humor drainage using QD nanoparticles and indocyanine green
Author(s) -
Nur İsmail Hakkı,
Saçmacı Şerife,
Orhan İmdat,
Perez William,
Testereci Haluk
Publication year - 2020
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23494
Subject(s) - indocyanine green , chemistry , in vivo , lymphatic system , biomedical engineering , saline , fluorescence , materials science , pathology , anatomy , medicine , optics , biology , physics , microbiology and biotechnology
This study represents the first morphological description of the lymphatic drainage of the ciliary body in vivo by comparative hyperspectral fluorescence imaging techniques of Quantum Qdot655 (QD) nanoparticles and indocyanine green (ICG). A volume of 1.25 μl of QD was injected into the left anterior camera of all rats. Similarly,1.25 μl of ICG diluted at a ratio of one‐fourth with physiological saline solution was injected into the right anterior camera of all rats. The thickness of the skin in the mandibular area, connective tissue, and the depth of the mandibular lymph node (MLN) made image retrieval difficult. For QD, 302 nm UV excitation and 605 nm fluorescence peak emission were applied. The detection of QD and ICG used in this study in the MLNs is definitive evidence that aqueous humor (AH) follows a uveolymphatic pathway. Scanning electron microscope and the energy dispersive X‐ray analyzer spectrum were used to examine both the Schlemm's canal and the MLN. For the first time, the QD was detected in the cortex of MLN. The QCM analysis of both QD‐AH and ICG‐AH was used to determine whether there was any interaction between them. This comparative study shows the importance of experimental animal modeling in pharmacological studies regarding eye research and drugs. In a female rat, the signal was taken from the parotid lymph node with QD injections.