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Sensitive protein detection and visualization using protein‐binding peptides
Author(s) -
Ma Lu,
Yang Tao,
Mao Chuanbin,
Yang Mingying
Publication year - 2020
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23482
Subject(s) - peptide , horseradish peroxidase , fluorescein isothiocyanate , chemistry , detection limit , substrate (aquarium) , silk , biophysics , fluorescence , biochemistry , chromatography , materials science , biology , enzyme , ecology , physics , quantum mechanics , composite material
Silk sericin (SS) as a silk protein has important applications in biomedical and textile fields, and thus finding a facile method for detecting and imaging it is very important. Here, we show that a free SS‐binding peptide, labeled with horseradish peroxidase and fluorescein isothiocyanate, could be used as a probe to determine the concentration of SS (from 1 to 100 μg/ml) in a dilute solution with a detection limit of 0.073 μg/ml and visualize the distribution of SS on a substrate under a fluorescent microscope, respectively. This work highlights the importance in discovering a protein‐binding peptide and the power of such a peptide for the detection and imaging of technologically important proteins.