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Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan
Author(s) -
Deroubaix Aurélie,
Moahla Bongani,
Penny Clement
Publication year - 2020
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23438
Subject(s) - intracellular , confocal , confocal microscopy , confocal laser scanning microscopy , laser microscopy , hepatitis a virus , microscopy , cytoplasm , virus , biology , virology , chemistry , microbiology and biotechnology , biophysics , pathology , optics , medicine , physics
The aim of this study was to assess nucleo‐cytoplasmic protein localization to better understand the exact intracellular localization of viral proteins involved with infections. Having determined the general protein localization of hepatitis B virus P22 precore protein, the aim was to more specifically resolve its intracellular organization. This was done using both laser scanning microscopy and Airyscan techniques. Using a 63× objective, the resolution obtained with Airyscan was increased by 1.5‐fold as compared to confocal microscopy ( p value <.00001).