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Taxonomic importance of spore morphology in Thelypteridaceae from Northern Pakistan
Author(s) -
Shah Syed N.,
Ahmad Mushtaq,
Zafar Muhammad,
Ullah Fazal,
Zaman Wajid,
Malik Khafsa,
Rashid Neelam,
Gul Saba
Publication year - 2019
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23283
Subject(s) - reticulate , subfamily , spore , morphology (biology) , taxon , botany , biology , principal component analysis , taxonomy (biology) , zoology , mathematics , statistics , gene , biochemistry
Spore morphology of Thelypteridaceae species growing in Malakand Division, Northern Pakistan, was studied using both light microscopy and scanning electron microscopy. The taxa are Christella dentata and Glaphyropteridopsis erubescens in the subfamily Thelypteridoideae, and Phegopteris connectilis , Pseudophegopteris pyrrhorhachis , and Pseudophegopteris levingei in the subfamily Phegopteridoideae. The studied species exhibit differences in spore size, exospore thickness, color, and ornamentation. Spores of the studied species are monolete and medium‐sized, and shape is ellipsoidal in both polar and equatorial views. The average measurement of the polar diameter ranges from 27 μm to 31 μm, whereas in the equatorial direction it varied from 20 μm to 40 μm. The exospore thickness ranges from 1.2 μm to 2.4 μm. Reticulate, laevigate with microgranules, cristate, and coarsely echinate surface ornamentation are observed among the species. Multivariate analysis including unweighted pair group method with arithmetic mean and principal component analysis was used for the grouping and discrimination of species and genera.