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Rapid histologic diagnosis using quick fluorescence staining and tissue confocal microscopy
Author(s) -
Kim Hongrae,
Lee Sunhye,
Kim Hyunjin,
Chang Hee Jin,
Choi Yongdoo,
Yoon Hong Man,
Kook MyeongCherl,
Jung SoYoun,
Kwon Youngmi,
Kang Juehyung,
Yoo Hongki,
Song Incheon,
Lee Seungrag,
Sohn Dae Kyung
Publication year - 2019
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23234
Subject(s) - staining , confocal microscopy , confocal , slicing , pathology , fluorescence microscope , microscopy , biomedical engineering , eosin , fluorescence , fluorescent staining , medicine , computer science , biology , optics , microbiology and biotechnology , physics , world wide web
With the development of advanced and minimally invasive surgical techniques, and in view of the functional and cosmetic aspects, the need for rapid and accurate diagnosis during surgery is increasing. This study was conducted to develop a tissue diagnosis method using confocal microscopy after simple tissue staining that does not require freezing and slicing. At present, fluorescence staining with confocal microscopy is not generalized for real‐time diagnosis during surgery. In this paper, we propose a fluorescence staining method using Hoechst 33342 and Eosin that does not require tissue freezing and slicing. The proposed method can be used as part of a rapid tissue diagnosis method that is suitable for use in the operating room, although further research is required before it can be applied in clinical practice.