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Effectiveness of rhBMP‐2 association to autogenous, allogeneic, and heterologous bone grafts
Author(s) -
Gonzaga Miliane Gonçalves,
Santos Kotake Bruna Gabriela,
Figueiredo Fellipe Augusto Tocchini,
Feldman Sara,
Ervolino Edilson,
Santos Maria Cecília Gorita,
Issa João Paulo Mardegan
Publication year - 2019
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23215
Subject(s) - bone morphogenetic protein 2 , bone morphogenetic protein , osteocalcin , immunostaining , bone sialoprotein , bone morphogenetic protein 7 , heterologous , calvaria , immunohistochemistry , vascular endothelial growth factor , chemistry , bone formation , andrology , medicine , vegf receptors , alkaline phosphatase , biochemistry , in vitro , gene , enzyme
Abstract Proteins with osteoinductive potential, especially recombinant human bone morphogenetic protein (rhBMP)‐2, have large effects on cell growth and their differentiation. The aim of this study was to assess repair of bone defects in rat calvaria with different types of grafts associated with rhBMP‐2, through immunohistochemistry and micro computed tomography (CT) analyses. A total of 35 male Wistar rats were selected, each weighing ~250 g, with a waiting period of 6 weeks from the creation of the defect to the sacrifice, and divided into five groups (n = 7): autograft plus 5 μg rhBMP‐2 (AuG/BMP‐2); allograft plus 5 μg rhBMP‐2 (AlG/BMP‐2); xenograft (heterologous) plus 5 μg rhBMP‐2 (XeG/BMP‐2); 5 μg rhBMP‐2 (BMP‐2) and the control group (n = 7). The micro CT reveal that all groups associating different bone grafts with BMP‐2 showed increased bone formation compared to the control. The immunostaining show that osteocalcin and bone sialoprotein were higher in groups with BMP‐2 than control group; BMP was high expressed in AuG/BMP‐2, AlG/BMP‐2, and BMP‐2; vascular endothelial growth factor (VEGF) was more expressed in groups with BMP‐2; VEGF‐R2 was low to moderate in AuG/BMP‐2, XeG/BMP‐2, and BMP‐2, predominantly moderate in AlG/BMP‐2 and low in the control; CD‐31 was predominantly moderate in AuG/BMP‐2, AlG/BMP‐2, and XeG/BMP‐2, low to moderate in BMP‐2 and low in the control. The results revealed that rhBMP‐2 improved bone repair when administered alone, or when associated with different bone grafts.