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3D measurements of micro‐objects based on monocular wide‐field optical microscopy with extended depth of field
Author(s) -
Qu Yufu,
Zhang Ping,
Hu Yongbo
Publication year - 2018
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23108
Subject(s) - monocular , depth of field , lens (geology) , microscopy , computer vision , artificial intelligence , computer science , optics , field (mathematics) , radius , microscope , field of view , optical microscope , stereoscopy , physics , mathematics , scanning electron microscope , computer security , pure mathematics
Abstract Monocular rotational microscopes suffer from shallow depth of field and reduced field of view. Consequently, we propose monocular wide‐field optical microscopy with extended depth of field to enable accurate 3D measurements of micro‐objects. We use a liquid lens and a 4f system to extend the system's depth of field and two additional rotational reflectors to capture multi‐view image sequences. In addition, we increase the number of expansion directions in the patch‐based multi‐view stereo algorithm and optimize the expansion radius. Our approach outperforms that based on the patch‐based multi‐view stereo method and achieves a high relative measurement accuracy of 1.9%. Research Highlights This study proposes 3D measurements of micro‐objects via monocular rotational microscopy. The depth of field is extended by the use of a liquid lens and a 4f system. Our approach outperforms that based on the patch‐based multi‐view stereo method and achieves a high relative measurement accuracy of 1.9%.