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Fusion of GFP and phase contrast images with complex shearlet transform and Haar wavelet‐based energy rule
Author(s) -
Qiu Chenhui,
Wang Yuanyuan,
Guo Yanen,
Xia Shunren
Publication year - 2018
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23012
Subject(s) - image fusion , artificial intelligence , contrast (vision) , fusion , wavelet , pattern recognition (psychology) , computer science , inverse , computer vision , fusion rules , wavelet transform , energy (signal processing) , contourlet , haar wavelet , image (mathematics) , discrete wavelet transform , mathematics , philosophy , linguistics , statistics , geometry
Image fusion techniques can integrate the information from different imaging modalities to get a composite image which is more suitable for human visual perception and further image processing tasks. Fusing green fluorescent protein (GFP) and phase contrast images is very important for subcellular localization, functional analysis of protein and genome expression. The fusion method of GFP and phase contrast images based on complex shearlet transform (CST) is proposed in this paper. Firstly the GFP image is converted to IHS model and its intensity component is obtained. Secondly the CST is performed on the intensity component and the phase contrast image to acquire the low‐frequency subbands and the high‐frequency subbands. Then the high‐frequency subbands are merged by the absolute‐maximum rule while the low‐frequency subbands are merged by the proposed Haar wavelet‐based energy (HWE) rule. Finally the fused image is obtained by performing the inverse CST on the merged subbands and conducting IHS‐to‐RGB conversion. The proposed fusion method is tested on a number of GFP and phase contrast images and compared with several popular image fusion methods. The experimental results demonstrate that the proposed fusion method can provide better fusion results in terms of subjective quality and objective evaluation.