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Effects of EDTA gel and chlorhexidine gel on root dentin permeability
Author(s) -
Bighetti Trevisan Rayana Longo,
Scatolin Renata Siqueira,
Castro Raucci Larissa Moreira Spinola de,
Raucci Neto Walter,
Froner Izabel Cristina
Publication year - 2018
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22963
Subject(s) - smear layer , ethylenediaminetetraacetic acid , dentin , dentinal tubule , chlorhexidine , saline , chemistry , dentistry , staining , scanning electron microscope , permeability (electromagnetism) , materials science , chelation , medicine , membrane , composite material , biochemistry , pathology , organic chemistry , endocrinology
The aim of this study was to evaluate the effect of 24% ethylenediaminetetraacetic acid (EDTA) gel and 2% chlorhexidine gel (CHX) in dentin permeability and smear layer removal from root canals instrumented with NiTi rotary system using histochemical staining and scanning electron microscopy (SEM) analysis. Overall, 43 premolars were classified into two experimental groups, EDTA ( n = 20) and CHX ( n = 20), and a negative control (NC) ( n = 3). All specimens were instrumented and the irrigant solutions were used after each file change. The EDTA group received a final rinse with 5‐ml 1% NaOCl followed by a 5‐ml 0.9% saline solution; the CHX group received a final rinse with 10‐ml 0.9% saline solution; and the negative control group received a final rinse with only 0.9% saline solution. Fifteen teeth from each group were prepared for histochemical staining and evaluation of dentin permeability using the image‐scanning software Axion Vision (v.4.8.2). Five remaining teeth were prepared for analysis using SEM for morphological analysis. The study found that 24% EDTA gel increased the permeability of dentin in all thirds evaluated and also demonstrated an increased cleaning ability, with dentinal walls free of smear layer and open dentinal tubules, as compared to 2% CHX gel. It was concluded that EDTA was efficient in cleaning the dentinal tubules and increased dentin permeability.