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Testing feasibility of an accurate microscopic assessment of macrovesicular steatosis in liver allograft biopsies by smartphone add‐on lenses
Author(s) -
Cesaretti Manuela,
Poté Nicolas,
Dondero Federica,
Cauchy François,
Schneck Anne Sophie,
Soubrane Olivier,
Paradis Valerie,
Diaspro Alberto,
Antonini Andrea
Publication year - 2018
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22956
Subject(s) - medicine , pathology , gold standard (test) , eyepiece , telepathology , biomedical engineering , radiology , telemedicine , biology , paleontology , lens (geology) , economic growth , health care , economics
Light microscopy is an essential tool in histological examination of tissue samples. However, the required equipment for a correct and rapid diagnosis is sometimes unavailable. Smartphones and mobile phone networks are widespread, and could be used for diagnostic imaging and telemedicine. Macrovesicular steatosis (MS) is a major risk factor for liver graft failure, and is only assessable by microscopic examination of a frozen tissue section. The aim of this study was to compare the microscopic assessment of MS in liver allograft biopsies by a smartphone with eyepiece adaptor (BLIPS device) to standard light microscopy. Forty liver graft biopsies were evaluated in transmitted light, using an Iphone 5s and 4 different mini‐objective, add‐on lenses. A significant correlation was reported between the two different approaches for graft MS assessment (Spearman's correlation coefficient: r s = 0.946; p < .001). Smartphone with eyepiece adaptor had similar discriminatory power to identify MS in liver grafts than standard light microscopy. Based on these findings, a smartphone integrated with a low‐cost eyepiece adaptor can achieve adequate accuracy in the assessment of MS in liver graft, and could be used as an alternative to standard light microscope when unavailable.