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Study of morphological and mechanical features of multinuclear and mononuclear SW480 cells by atomic force microscopy
Author(s) -
Liu Jinyun,
Qu Yingmin,
Wang Guoliang,
Wang Xinyue,
Zhang Wenxiao,
Li Jingmei,
Wang Zuobin,
Li Dayou,
Jiang Jinlan
Publication year - 2018
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22950
Subject(s) - peripheral blood mononuclear cell , atomic force microscopy , cancer cell , cell , chemistry , cell culture , biophysics , cancer , microbiology and biotechnology , materials science , in vitro , medicine , biochemistry , biology , nanotechnology , genetics
Abstract This article studies the morphological and mechanical features of multinuclear and mononuclear SW480 colon cancer cells by atomic force microscopy to understand their drug‐resistance. The SW480 cells were incubated with the fullerenol concentrations of 1 mg/ml and 2 mg/ml. Morphological and mechanical features including the height, length, width, roughness, adhesion force and Young's modulus of three multinuclear cell groups and three mononuclear cell groups were imaged and analyzed. It was observed that the features of multinuclear cancer cells and mononuclear cancer cells were significantly different after the treatment with fullerenol. The experiment results indicated that the mononuclear SW480 cells were more sensitive to fullerenol than the multinuclear SW480 cells, and the multinuclear SW480 cells exhibited a stronger drug‐resistance than the mononuclear SW480 cells. This work provides a guideline for the treatments of multinuclear and mononuclear cancer cells with drugs.