Premium
Site‐specific accumulation and dynamic change of flavonoids in Apocyni Veneti Folium
Author(s) -
Chen CuiHua,
Xu Hu,
Liu XunHong,
Zou LiSi,
Wang Mei,
Liu ZiXiu,
Fu XingSheng,
Zhao Hui,
Yan Ying
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22943
Subject(s) - folium of descartes , kaempferol , chemistry , quercetin , phloem , botany , staining , flavonols , biochemistry , biology , high performance liquid chromatography , chromatography , genetics , antioxidant
Site‐specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin ‐HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured using HPLC method. LSCM observation showed that flavonoids are accumulated in cuticle of epidermal cells and vessel walls, especially in protoplasts and nucleolus of the collenchyma cells and the epidermal cells. Catechins are localized in the palisade parenchyma cells and vessel walls, particularly in the laticifers found in the phloem. On the basis of the difference of the maximal emission wavelength between quercetin and kaempferol derivatives which have fluorescence behavior by appropriate treatment, kaempferol and its derivatives are localized exclusively in the cuticle. Results showed that the content of astragalin in Apocyni Veneti Folium from different parts revealed the decreasing trend, while hyperin and isoquercitrin were higher in June and July analyzed by HPLC. In summary, the site‐specific accumulation of flavonoids in Apocyni Veneti Folium can be determined by LSCM and vanillin ‐HCl staining. The contents of flavonoids in Apocyni Veneti Folium are correlated with harvest times and growth parts.