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Effect of Chenopodium ambrosioides on the healing process of the in vivo bone tissue
Author(s) -
Penha Elizandra Silva da,
LacerdaSantos Rogério,
Carvalho Maria Goretti Freire de,
Oliveira Patrícia Teixeira de
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22913
Subject(s) - bone healing , in vivo , fibrin , wound healing , chemistry , medicine , pathology , andrology , anatomy , surgery , biology , immunology , microbiology and biotechnology
The focus of this double‐blind randomized study was on evaluating the effect of an aqueous extract of Mastruz ( Chenopodium ambrosioides L.) on the bone repair process in vivo. In total, 36 male Wistar rats were randomly selected for this study, and divided into 3 groups ( n = 12): Group HS (Hemostatic Sponge), Group SM (Hemostatic Sponge with Mastruz) and Group BC (Blood Clot). In each animal, bone defects measuring 2 mm in diameter were performed in both tibias for placement of the substances. After 3 and 10 days, the animals were sacrificed, and the tissues were analyzed under an optical microscope relative to the following events: inflammatory infiltrate; necrosis; young fibroblasts; osteoclastic and osteoblastic activity; endosteal and periosteal bone formation; and bone repair. The results were assessed by using Kruskal–Wallis and Mann–Whitney tests ( p < .05). Inflammatory infiltrate demonstrated difference between Groups SM and BC in the time interval of 3 days ( p = .004); an event related to the presence of the fibrin sponge and liquid of the extract, which induced a foreign body initial reaction. The presence of young fibroblasts ( p = .003), osteoclastic ( p = .003), and osteoblastic ( p = .020) activity was statistically significant between Groups HS and BC in the time interval of 10 days; performance was related to the presence of the sponge within bone. As regards injured bone tissue repair, Group SM demonstrated a higher level of regenerative capacity ( p = 0.004), due to a larger quantities of endosteal and periosteal bone formation, demonstrated in Group SM. The aqueous extract of mastruz stimulated bone neoformation, presenting wound closure with bone tissue at the end of 10 days.