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A new method using Scanning Electron Microscopy (SEM) for preparation of anisopterous odonates
Author(s) -
del Palacio Alejandro,
Sarmiento Patricia Laura,
Javier Muzón
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22903
Subject(s) - scanning electron microscope , electron microscope , biology , materials science , nanotechnology , zoology , optics , physics , composite material
Anisopterous odonate male's secondary genitalia is a complex of several structures, among them the vesica spermalis is the most informative with important specific characters. The observation of those characters, mostly of membranous nature, is difficult in the Scanning Electron Microscope due to dehydration and metallization processes. In this contribution, we discuss a new and low cost procedure for the observation of these characters in the SEM, compatible with the most common agents used for preserving specimens.