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The cardiovascular functions of salusin‐β mediated by muscarinic receptors, glutamate receptors or L‐type calcium channels within the rostral ventrolateral medulla of rats
Author(s) -
Xie FuJia,
Chai Chen,
Zhu Ping,
Li Bin,
Cai HongYan,
Lu Yan,
Cao g
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22868
Subject(s) - rostral ventrolateral medulla , microinjection , medicine , endocrinology , muscarinic acetylcholine receptor , glutamate receptor , microinjections , autonomic nervous system , receptor , oxotremorine , chemistry , medulla oblongata , heart rate , blood pressure , central nervous system
Salusin‐α and salusin‐β are newly identified bioactive peptides of 28 and 20 amino acids, respectively, that were initially predicted using in silico analyses and are widely distributed in the endocrine system, hematopoietic system, and central nervous system. The goal of our study was to investigate the cardiovascular effect of salusin‐β microinjections into the rostral ventrolateral medulla (RVLM) in anesthetized rats and study their mechanism of action. Microinjection of the artificial cerebrospinal fluid (aCSF) into the RVLM did not affect the blood pressure (BP) or heart rate (HR) in anesthetized rats. Topical application of salusin‐β into the RVLM produced a dose‐dependently increase of BP in anesthetized rats. Microinjection of higher dose salusin‐β produced significant tachycardia. Prior application of the L‐NAME into the RVLM of rats did not alter the hypertension and tachycardia induced by intra‐RVLM salusin‐β. Notable, the cardiovascular functions elicited by intra‐RVLM salusin‐β were significantly decreased by pretreatment with Nic, KYN and atropine. In conclusion, the present study shows that the hypertension and tachycardia induced by intra‐RVLM salusin‐β might be partly mediated, at least in our opinion, by muscarinic receptors, glutamate receptors or L‐type calcium channels.