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Dimethyl‐2‐[(acridin‐9‐yl)methylidene]‐malonate as fluorescent probe for histochemical analysis
Author(s) -
Almeida Sinara Mônica Vitalino,
Silva Lúcia Patrícia Bezerra Gomes,
Lima Luiza Rayanna Amorim,
Botelho Sandra Paula Sarinho,
Lima Maria do Carmo,
Pitta Ivan da Rocha,
Beltrão Eduardo Isidoro Carneiro,
Carvalho Júnior Luiz Bezerra
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22837
Subject(s) - lectin , fluorescence , conjugate , malonate , derivative (finance) , biology , chemistry , biochemistry , mathematical analysis , physics , mathematics , quantum mechanics , financial economics , economics
Abstract Fluorescent compounds have been widely used for biomolecule labeling in cytochemistry and histochemistry analysis. Here, it is described the optical properties of dimethyl 2‐[(acridin‐9‐yl)methylidene]‐malonate (LPSF/IP‐81), an acridine derivative. This compound was conjugated to Concanavalin A (Con A) lectin and applied as sugar probe in lectin histochemistry. Evaluation of luminescent properties showed that LPSF/IP‐81 is photoluminescent with excitation at 360 nm and emission at 428 nm. Con A hemagglutinating activity and LPSF/IP81 photoluminescence were unaltered after conjugation. Circular dichroism of Con A‐LPSF/IP81 conjugate showed the maintenance of the Con A structure. Lectin histochemistry with Con A‐LPSF/IP81 conjugate demonstrated different pattern recognition studying normal, fibroadenoma, and invasive ductal carcinoma of human breast. These findings indicate that LPSF/IP‐81 can be proposed as an alternative probe for histochemical analysis.