Premium
Comparison of manual and automatic processing of biological samples for electron microscopy
Author(s) -
Yang Rui,
Wang Shuai,
Wang Jianli,
Luo Xi,
Zhao Wenchao,
Zhang Qing,
Nan Zhangjie,
Yang Liu,
Wang Shaohui
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22832
Subject(s) - sample (material) , electron microscope , sample preparation , microscopy , biological specimen , ultrastructure , scanning electron microscope , computer science , transmission electron microscopy , materials science , nanotechnology , chemistry , optics , biology , chromatography , physics , composite material , anatomy
The ultrastructure of a sample can be observed by electron microscopy (EM), which has become an indispensable research tool in morphological studies. However, EM sample preparation techniques are complicated and time‐consuming, with a high labor cost. The current study was conducted to compare the conventional manual and automated methods for sample processing and post‐staining for electron microscopy. Automated sample processing reduces OsO 4 contamination, improves the efficiency of sample preparation and is easy to use. Therefore, the results of their study provide a practical and feasible method for the preparation of biological samples for electron microscopy.