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Transmission electron microscopy method providing high resolution in the cell section without radiation damage
Author(s) -
Prachař Jarmil
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22813
Subject(s) - resolution (logic) , transmission electron microscopy , section (typography) , nucleoid , transmission (telecommunications) , microscopy , high resolution , optics , electron microscope , work (physics) , image resolution , physics , materials science , computer science , biology , remote sensing , geology , telecommunications , artificial intelligence , genetics , thermodynamics , escherichia coli , gene , operating system
Several new features of mitochondrial nucleoid and its surroundings in mammalian cells were described previously (Prachař, 2016). Very small details were observed using the improved transmission electron microscopy method, as described in the article. In the meantime, the method has again been improved to 2 Å resolutions in the cell section. The method described in detail in the present work is documented on the same records that were published in lower resolution in the work Prachař (2016), enabling comparison of the achieved resolution with the previous one. New records are also presented, showing extremely high resolution and thus implying the importance of the method. Potential use of this method in different fields is suggested.