Premium
Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles
Author(s) -
Lima Lartiza F.,
Rocha Rebeca M. P.,
Duarte Ana Beatriz G.,
Brito Ivina R.,
Silva Gerlane M.,
Rodrigues Giovanna Q.,
NunesPinheiro Diana C. S.,
Sales Antônia D.,
Moura Arlindo A.,
Wheeler Matthew B.,
Rodrigues Ana Paula R.,
Campello Cláudio C.,
Figueiredo José Ricardo
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22810
Subject(s) - in vitro , ethanol , andrology , homeopathy , biology , chemistry , biochemistry , medicine , pathology , alternative medicine
The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic – FSH 6cH and allopathic FSH – rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α‐MEM + supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved ( p < .05) follicular development compared to rFSH100 treatment without addition. FSH 6cH with addition showed the highest ( p < .05) estradiol production. To verify whether the effects of homeopathic FSH were not due to its vehicle, experiment II was performed. The α‐MEM + was supplemented or not with alcohol (0.2% grain ethanol, v/v), FSH 6cH or rFSH100 with daily medium addition. Surprisingly, we found that all treatments improved follicular development compared to the α‐MEM + ( p < .05). Moreover, homeopathic FSH was similar to the other treatments including its vehicle. In conclusion, its vehicle (ethanol) causes the effect of homeopathic FSH on in vitro development of isolated ovine preantral follicles.