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Anthera ea pernyi silk sericin mediating biomimetic nucleation and growth of hydroxylapatite crystals promoting bone matrix formation
Author(s) -
Jiayao Zhuang,
Guanshan Zhou,
Jinchi Zhang,
Yuyin Chen,
Yongqiang Zhu
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22793
Subject(s) - antheraea pernyi , biomineralization , sericin , nucleation , silk , chemical engineering , chemistry , extracellular matrix , biophysics , materials science , hydroxylapatite , tissue engineering , biochemistry , biomedical engineering , composite material , organic chemistry , biology , medicine , gene , enzyme , engineering
Bone biomineralization is well‐regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well‐structured material‐minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi ( A.pernyi ), non‐mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations. The HAp crystals are organized into nano‐rods oriented with c‐axis preferentially parallel to the long axis of AS due to hydrogen bonds and electrostatic interaction and finally aggregated into HAp globule. The cell culture of human bone marrow‐derived mesenchymal stem cells (BMSCs) showed that the HAp crystals mediated by AS not only stimulate cell adhesion and proliferation but also promote 0f osteogenic differentiation, suggesting that the resultant mineralized AS biomaterial has potential in promoting bone formation. Thus our work will provide significant implication on biomineralization of A. pernyi silk sericin as a potential scaffold for tissue engineering.