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Time influence on the interaction between Cyt2Aa2 and lipid/cholesterol bilayers
Author(s) -
MorenoCencerrado Alberto,
Tharad Sudarat,
Iturri Jagoba,
Promdonkoy Boonhiang,
Krittanai Chartchai,
TocaHerrera José L.
Publication year - 2016
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22736
Subject(s) - lipid bilayer , biophysics , chemistry , bilayer , lipid bilayer phase behavior , atomic force microscopy , molecular dynamics , cholesterol , protein–protein interaction , membrane protein , force spectroscopy , membrane , biochemistry , nanotechnology , materials science , biology , computational chemistry
Protein–membrane interactions are still an important topic of investigation. One of the suitable experimental techniques used by the scientific community to address such question is atomic force microscopy. In a previous work, we have reported that the binding mechanism between the cytolytic and antimicrobial protein (Cyt2Aa2) and lipid/cholesterol bilayers was concentration‐dependent, leading to either the formation of holes in the bilayer or aggregates. Here we study such binding mechanism as a function of time at low protein concentrations (10 µg/mL). We demonstrate that although holes are formed during the first stages of the protein–lipid interaction, a reparation process due to molecular mobility in the bilayer leads to a homogenous and isotropic protein–lipid/cholesterol layer within 3 hr. The combination of imaging, force spectroscopy, and phase contrast delivered information about topography dynamics (molecular mobility), layer thickness, and mechanical properties of the protein–lipid/cholesterol system. These results highlight the importance of the observation time in (such type of) protein–lipid interactions (at low protein concentrations).