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Albumin microvascular leakage in brains with diabetes mellitus
Author(s) -
Fujihara Ryuji,
Chiba Yoichi,
Nakagawa Toshitaka,
Nishi Nozomu,
Murakami Ryuta,
Matsumoto Koichi,
Kawauchi Machi,
Yamamoto Tetsuji,
Ueno Masaki
Publication year - 2016
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22708
Subject(s) - albumin , spinal cord , white matter , diabetes mellitus , blood–brain barrier , medicine , endocrinology , vascular permeability , hyperintensity , endogeny , pathology , magnetic resonance imaging , central nervous system , psychiatry , radiology
ABSTRACT Their aim was to examine whether microvascular leakage of endogenous albumin, a representative marker for blood–brain barrier (BBB) damage, was induced in the periventricular area of diabetic db/db mice because periventricular white matter hyperintensity formation in magnetic resonance images was accelerating in elderly patients with diabetes mellitus. Using light and electron microscopes, and semi‐quantitative analysis techniques, immunoreactivity of endogenous albumin, indicating vascular permeability, was examined in the periventricular area and spinal cord of db/db mice and db/+m control mice. Greater immunoreactivity of albumin was observed in the vessel wall of the periventricular area of db/db mice than in controls. Additionally, weak immunoreactivity was observed in the spinal cord of both db/db mice and controls. The number of gold particles, indicating immunoreactivity of albumin, in the perivascular area of db/db mice was significantly higher than that of control mice, but there was no significant difference in the number of particles in the spinal cord between db/db mice and controls. These findings suggest that albumin microvascular leakage, or BBB breakdown, is induced in the periventricular area of diabetic mice. Microsc. Res. Tech. 79:833–837, 2016 . © 2016 Wiley Periodicals, Inc.