Premium
Biotinylated vanadium and chromium sulfide nanoparticles as probes for colocalization of membrane proteins
Author(s) -
Loukanov Alexandre,
Emin Saim
Publication year - 2016
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22701
Subject(s) - chemistry , nanoparticle , microemulsion , vanadium , sodium dodecyl sulfate , biotinylation , inorganic chemistry , aqueous solution , membrane , aqueous two phase system , nuclear chemistry , chemical engineering , chromatography , pulmonary surfactant , organic chemistry , biochemistry , engineering
We report the microemulsion synthesis of vanadium and chromium sulfide nanoparticles (NPs) and their biological application as nanoprobes for colocalization of membrane proteins. Spherical V 2 S 3 and Cr 2 S 3 NPs were prepared in reverse microemulsion droplets, as nanoreactors, obtained by the surfactant sodium bis(2‐ethylhexyl) sulfosuccinate (AOT) in nonpolar organic phase (heptane). Electron microscopic data indicated that the size distribution of the nanoparticles was uniform with an average diameter between 3 ÷ 5 nm. The prepared hydrophobic nanocrystals were transferred in aqueous phase by surface cap exchange of AOT with biotin‐dihydrolipoic ligands. This substitution allows the nanoparticles solubility in aqueous solutions and confer their bioactivity. In addition, we report the conjugation procedure between α‐ Lipoic acid (LA) and biotin (abbreviated as b iotin‐LA ). The biotin‐LA structure was characterized by 1D and 2D NMR spectroscopy. The biotinylated vanadium and chromium sulfide nanoparticles were tested as probes for colocalization of glutamate receptors on sodium‐dodecyl‐sulfate‐digested replica prepared from rat hippocampus. The method suggests their high labeling efficiency for study of membrane biological macromolecules. Microsc. Res. Tech. 79:799–805, 2016 . © 2016 Wiley Periodicals, Inc.