Premium
In situ cultured preantral follicles is a useful model to evaluate the effect of anticancer drugs on caprine folliculogenesis
Author(s) -
Guerreiro Denise Damasceno,
Lima Laritza Ferreira de,
Rodrigues Giovanna Quintino,
Carvalho Adeline. de Andrade,
Castro Simone Vieira,
Campello Cláudio Cabral,
Pessoa Cláudia do Ó,
Gadelha Carla Renata Figueiredo,
Figueiredo José Ricardo de,
Bordig Vilceu,
Rodrigues Ana Paula Ribeiro
Publication year - 2016
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22697
Subject(s) - folliculogenesis , biology , oogenesis , paclitaxel , apoptosis , dna laddering , ovarian follicle , in vitro , premature ovarian failure , tunel assay , ovarian cancer , andrology , ovary , medicine , endocrinology , oocyte , chemotherapy , cancer , dna fragmentation , microbiology and biotechnology , embryogenesis , programmed cell death , biochemistry , embryo , genetics
Despite the increase in the incidence of cancer, the number of women who survive cancer treatment is growing. However, one of the principal results of chemotherapy is premature ovarian failure (POF). The aim of this study was to use the in situ culture preantral follicles as an in vitro model to evaluate the toxicity of two anticancer drugs, doxorubicin (DXR) and paclitaxel (PTX), on the integrity and development of ovarian follicles. Fragments of the ovarian cortex of goats were cultured in vitro for 1 or 7 days in α‐MEM + supplemented with different concentrations of DXR (0.003, 0.03, or 0.3 µg/mL) and PTX (0.001, 0.01, or 0.1 µg/mL). Analyses were performed before and after culture to evaluate tissue integrity by classical histology, apoptosis by TUNEL assay, DNA laddering kit and the detection of activated caspase 3, and DNA damage by the immune detection of phosphorylated histone H2A.x (H2AXph139). Both DXR and PTX reduced the number of morphologically normal primordial and developing follicles. Positive staining for TUNEL and active caspase 3 was detected in all the samples ( P < 0.05). Therefore, we propose the in situ culture of caprine preantral follicles as a useful experimental model for assessing the toxic effects of the chemotherapeutic agents on ovarian folliculogenesis. Microsc. Res. Tech. 79:773–781, 2016 . © 2016 Wiley Periodicals, Inc.