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Mapping phase diagrams of supported lipid bilayers by atomic force microscopy
Author(s) -
Borrell Jordi H.,
Montero M. Teresa,
Domènech Òscar
Publication year - 2017
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22655
Subject(s) - phospholipid , phase diagram , mica , lipid bilayer , atomic force microscopy , chemistry , phase (matter) , liposome , substrate (aquarium) , phase transition , crystallography , chemical engineering , analytical chemistry (journal) , materials science , membrane , nanotechnology , chromatography , thermodynamics , organic chemistry , geology , composite material , physics , biochemistry , oceanography , engineering
ABSTRACT In this work, we present the method followed to construct a pseudophase diagram of two phospholipids: 1‐palmitoyl‐2‐oleoyl‐ sn ‐glycero‐3‐phosphoethanolamine and 1‐palmitoyl‐2‐oleoyl‐ sn ‐glycero‐3‐phospho‐(1′‐ rac ‐glycerol). Two different techniques, DSC and AFM, have been used based in the determination of the onset ( T onset ) and completion ( T offset ) temperatures of the gel‐to‐liquid crystalline phases ( L β →L α ), the first from the endotherms from liposomes and the second from the topographic images of supported lipid bilayers. The features of both phase diagrams are discussed emphasizing the influence of Ca 2+ presence and the substrate (mica) on the transition undergone by the phospholipid mixture. Microsc. Res. Tech. 80:4–10, 2017 . © 2016 Wiley Periodicals, Inc.