Premium
Multiphoton imaging with a novel compact diode‐pumped T i:sapphire oscillator
Author(s) -
König Karsten,
Andersen Peter,
Le Tuan,
Breunig Hans Georg
Publication year - 2015
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22599
Subject(s) - sapphire , laser , femtosecond , materials science , optics , optoelectronics , diode , ti:sapphire laser , distributed bragg reflector , microscopy , physics
Multiphoton laser scanning microscopy commonly relies on bulky and expensive femtosecond lasers. We integrated a novel minimal‐footprint Ti:sapphire oscillator, pumped by a frequency‐doubled distributed Bragg reflector tapered diode laser, into a clinical multiphoton tomograph and evaluated its imaging capability using different biological samples, i.e. cell monolayers, corneal tissue, and human skin. With the novel laser, the realization of very compact Ti:sapphire‐based systems for high‐quality multiphoton imaging at a significantly size and weight compared to current systems will become possible. Microsc. Res. Tech. 78:1154–1158, 2015 . © 2015 Wiley Periodicals, Inc.