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Depletion of enteroendocrine and mucus‐secreting cells is associated with colorectal carcinogenesis severity and impaired intestinal motility in rats
Author(s) -
Novaes Rômulo Dias,
Sequetto Priscila Lima,
Vilela Gonçalves Reggiani,
Cupertino Marli Carmo,
Santos Eliziária Cardoso,
Mello Vanessa Joia,
Araújo Marta Rocha,
Silva Edson,
Oliveira Tânia Toledo
Publication year - 2016
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22534
Subject(s) - motility , enteroendocrine cell , mucus , intestinal motility , carcinogenesis , endocrine system , distal colon , biology , medicine , mucin , cancer research , endocrinology , pathology , microbiology and biotechnology , cancer , hormone , ecology
This study investigated the relationship between enteroendocrine and mucus‐secreting cells distribution, the severity of colonic mucosal injury and intestinal motility in experimental colorectal carcinogenesis. Using a standardized murine model of colorectal carcinogenesis, eight‐weeks‐old female Wistar rats weighting 147.30 ± 29.15g were randomized into two groups receiving a subcutaneous injection of 0.9% saline (control) or the chemical carcinogen 1,2‐dimethylhydrazine (DMH) at 20 mg/kg per week during 10 weeks. Aberrant crypt foci (ACF) were more frequent in DMH group compared to control group ( P < 0.001). The number of enteroendocrine and mucus‐secreting cells, and intestinal motility was reduced in DMH animals ( P < 0.05). The distribution of enteric neurons was similar in both groups. In DMH animals there was a direct correlation between colonic motility and distribution of enteroendocrine ( R 2 = 0.68, P < 0.05) and mucus‐secreting cells ( R 2 = 0.77, P < 0.05). Inverse correlation between the number of ACF, mucus‐secreting cells ( R 2 = −0.57, P < 0.05), and enteroendocrine cells ( R 2 = −0.74, P < 0.05) was also observed. There was inverse correlation between the severity of the mucosal lesion, the number of mucus‐secreting cells ( R 2 = −0.83, P < 0.05) and enteroendocrine cells ( R 2 = −0.96, P < 0.05). There was a direct correlation between nucleolar organizer regions (AgNOR) and ACF number ( R 2 = 0.62; P < 0.01). Inverse correlation was also found between AgNOR, the number of mucus‐secreting cells ( R 2 = −0.76; P < 0.001), and enteroendocrine cells ( R 2 = −0.86; P < 0.001). Taken together, the results indicated that colonic malignant transformation is related to depletion of mucus‐secreting and enteroendocrine cells, which was a useful indicator of the evolutionary status of intestinal neoplasm, partially explaining the intestinal motility disorders in the early stages of colorectal carcinogenesis. Microsc. Res. Tech. 79:3–13, 2016 . © 2015 Wiley Periodicals, Inc.