Premium
Different bacterial models for in vitro induction of non‐cavitated enamel caries‐like lesions: Microhardness and polarized light miscroscopy analyses
Author(s) -
De Campos Priscila Hernández,
Sanabe Mariane Emi,
Rodrigues Jonas Almeida,
Duarte Danilo Antonio,
Santos Maria Teresa Botti Rodrigues,
Guaré Renata Oliveira,
Duque cristiane,
Lussi Adrian,
Diniz Michele Baffi
Publication year - 2015
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22493
Subject(s) - enamel paint , streptococcus mutans , lactobacillus acidophilus , lactobacillus casei , in vitro , dentistry , polarized light microscopy , chemistry , lesion , medicine , bacteria , biology , pathology , food science , biochemistry , probiotic , genetics , physics , fermentation , optics
The aim of this study was to compare different bacterial models for in vitro induction of non‐cavitated enamel caries‐like lesions by microhardness and polarized light microscopy analyses. One hundred blocks of bovine enamel were randomly divided into four groups ( n = 25) according to the bacterial model for caries induction: (A) Streptococcus mutans , (B) S. mutans and Lactobacillus acidophilus , (C) S. mutans and L. casei , and (D) S. mutans, L. acidophilus , and L. casei . Within each group, the blocks were randomly divided into five subgroups according to the duration of the period of caries induction (4–20 days). The enamel blocks were immersed in cariogenic solution containing the microorganisms, which was changed every 48 h. Groups C and D presented lower surface hardness values (SMH) and higher area of hardness loss (Δ S ) after the cariogenic challenge than groups A and B ( P < 0.05). As regards lesion depth, under polarized light microscopy, group A presented significantly lower values, and groups C and D the highest values. Group B showed a higher value than group A ( P < 0.05). Groups A and B exhibited subsurface caries lesions after all treatment durations, while groups C and D presented erosion‐type lesions with surface softening. The model using S. mutans , whether or not it was associated with L. acidophilus , was less aggressive and may be used for the induction of non‐cavitated enamel caries‐like lesions. The optimal period for inducing caries‐like lesions was 8 days. Microsc. Res. Tech. 78:444–451, 2015 . © 2015 Wiley Periodicals, Inc.