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Laser‐induced shockwave paired with FRET : A method to study cell signaling
Author(s) -
GomezGodinez Veronica,
Preece Daryl,
Shi Linda,
Khatibzadeh Nima,
Rosales Derrick,
Pan Yijia,
Lei Lie,
Wang Yingxiao,
Berns Michael W.
Publication year - 2015
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22463
Subject(s) - förster resonance energy transfer , biosensor , biophysics , chemistry , intracellular , laser , confocal , nanotechnology , microbiology and biotechnology , fluorescence , materials science , biology , physics , optics , biochemistry
Cells within the body are subject to various forces; however, the details concerning the way in which cells respond to mechanical stimuli are not well understood. We demonstrate that laser‐induced shockwaves (LIS) combined with biosensors based on fluorescence resonance energy transfer (FRET) is a promising new approach to study biological processes in single live cells. As “proof‐of‐concept,” using a FRET biosensor, we show that in response to LIS, cells release intracellular calcium. With the parameters used, cells retain their morphology and remain viable. LIS combined with FRET permits observation of the cells immediate response to a sudden shear force. Microsc. Res. Tech. 78:195–199, 2015. © 2015 Wiley Periodicals, Inc.