Premium
Budding process during the organogenesis of the ventral prostatic lobe in mongolian gerbil
Author(s) -
Sanches Bruno Domingos Azevedo,
Biancardi Manoel Francisco,
Santos Fernanda Cristina Alcantara,
GóEs Rejane Maira,
Vilamaior PatríCia Simone Leite,
Taboga SebastiãO Roberto
Publication year - 2014
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22370
Subject(s) - budding , biology , mesenchyme , organogenesis , prostate , androgen receptor , anatomy , pathology , microbiology and biotechnology , mesenchymal stem cell , medicine , prostate cancer , biochemistry , genetics , cancer , gene
The prostate is a mammalian gland that shows a complex process of organogenesis. Here, a morphological study to characterize the organogenesis of the ventral prostate lobe in male gerbils was conducted. The urogenital sinus (UGS) was dissected out and processed for paraffin embedding. Histological sections were subjected to cytochemical, immunofluorescence, immunohistochemical, and three‐dimensional reconstruction techniques. We found that the first ventral buds emerged from the ventral urethral epithelium between the days 20 and 21 of prenatal life, reaching the ventral mesenchymal pad and initiating the branching process on the first day of postnatal life. The buds presented a V‐shaped elongation, suggesting that the smooth muscle layer (SML) plays an important role during budding events. Indeed, whereas the androgen receptor (AR) was preferentially found in the UGS mesenchyme (UGM), estrogen receptor alpha (ERα) was localized in both the UGM and in the emerging buds. This study characterized the morphological aspects of the budding process in a different rodent from rat and mice, serving as a new model for future studies on developmental biology of the prostate. Microsc. Res. Tech. 77:458–466, 2014 . © 2014 Wiley Periodicals, Inc.