Premium
Single‐molecule super‐resolution imaging by tryptophan‐quenching‐induced photoswitching of phalloidin‐fluorophore conjugates
Author(s) -
Nanguneri Siddharth,
Flottmann Benjamin,
Herrmannsdörfer Frank,
Thomas Kuner,
Heilemann Mike
Publication year - 2014
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22349
Subject(s) - fluorophore , chemistry , phalloidin , fluorescence , context (archaeology) , biological imaging , molecule , quenching (fluorescence) , conjugate , photochemistry , biophysics , nanotechnology , materials science , optics , biochemistry , organic chemistry , paleontology , physics , cytoskeleton , biology , cell , mathematical analysis , mathematics
Photophysical properties of any fluorophore are governed by the chemical nanoenvironment. In the context of imaging biological samples, this translates to different photophysical properties for different labels and probes. Here, we demonstrate that the nanoenvironment of fluorophores within a probe can be advantageously used to induce particular properties such as light‐induced photoswitching. We demonstrate efficient photoswitching and single‐molecule super‐resolution imaging for various fluorophore‐phalloidin conjugates in aqueous buffer without the addition of further chemicals. We further demonstrate the utility of two‐color imaging of fluorophore‐phalloidin and a photoactivatable fluorescent protein in presynaptic nerve terminals. Microsc. Res. Tech. 77:510–516, 2014 . © 2014 Wiley Periodicals, Inc.