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Super‐resolution imaging with stochastic single‐molecule localization: Concepts, technical developments, and biological applications
Author(s) -
Oddone Anna,
Vilanova Ione Verdeny,
Tam Johnny,
Lakadamyali Melike
Publication year - 2014
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22346
Subject(s) - nanotechnology , biological imaging , resolution (logic) , enthusiasm , microscopy , computer science , data science , optics , physics , philosophy , materials science , artificial intelligence , fluorescence , theology
Light microscopy has undergone a revolution with the advent of super‐resolution microscopy methods that can surpass the diffraction limit. These methods have generated much enthusiasm, in particular with regards to the new possibilities they offer for biological imaging. The recent years have seen a great advancement both in terms of new technological developments and exciting biological applications. Here, we review some of the important milestones in the field and highlight some recent biological applications. Microsc. Res. Tech. 77:502–509, 2014 . © 2014 Wiley Periodicals, Inc.