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Light sheet based imaging flow cytometry on a microfluidic platform
Author(s) -
regmi Raju,
Mohan Kavya,
Mondal Partha P.
Publication year - 2013
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22296
Subject(s) - microfluidics , laminar flow , flow cytometry , cell counting , flow focusing , cytometry , flow (mathematics) , throughput , volumetric flow rate , nanotechnology , materials science , biomedical engineering , optics , computer science , chemistry , physics , mathematics , engineering , cell , biology , geometry , cell cycle , genetics , telecommunications , biochemistry , quantum mechanics , wireless , thermodynamics
We propose a light sheet based imaging flow cytometry technique for simultaneous counting and imaging of cells on a microfluidic platform. Light sheet covers the entire microfluidic channel and thus omits the necessity of flow focusing and point scanning based technology. Another advantage lies in the orthogonal detection geometry that totally cuts‐off the incident light, thereby substantially reducing the background in the detection. Compared to the existing state‐of‐art techniques the proposed technique shows marked improvement. Using fluorescently‐coated Saccharomyces cerevisiae cells we have recorded cell counting with throughput as high as 2,090 cells/min in the low flow rate regime and were able to image the individual cells on‐the‐go. Overall, the proposed system is cost‐effective and simple in channel geometry with the advantage of efficient counting in operational regime of low laminar flow. This technique may advance the emerging field of microfluidic based cytometry for applications in nanomedicine and point of care diagnostics. Microsc. Res. Tech. 76:1101–1107, 2013 . © 2013 Wiley Periodicals, Inc.