Premium
Phase contrast without phase plates and phase rings—optical solutions for improved imaging of phase structures
Author(s) -
Piper Timm,
Piper Jörg
Publication year - 2013
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22265
Subject(s) - optics , phase (matter) , brightness , phase contrast microscopy , materials science , microscope , optical microscope , concentric , microscopy , phase contrast imaging , contrast (vision) , resolution (logic) , physics , mathematics , scanning electron microscope , geometry , computer science , quantum mechanics , artificial intelligence
Using the optical methods described, phase specimens can be observed with a modified light microscope in enhanced clarity, purified from typical artifacts which are apparent in standard phase contrast illumination. In particular, haloing and shade‐off are absent, lateral and vertical resolution are maximized and the image quality remains constant even in problematic preparations which cannot be well examined in normal phase contrast, such as specimens beyond a critical thickness or covered by obliquely situated cover slips. The background brightness and thus the range of contrast can be continuously modulated and specimens can be illuminated in concentric‐peripheral, axial or paraxial light. Additional contrast effects can be achieved by spectral color separation. Normal glass or mirror lenses can be used; they do not need to be fitted with a phase plate or a phase ring. The methods described should be of general interest for all disciplines using phase microscopy. Microsc. Res. Tech., 76:1050–1056, 2013 . © 2013 Wiley Periodicals, Inc.