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Mesoporous iron oxide nanoparticles prepared by polyacrylic acid etching and their application in gene delivery to mesenchymal stem cells
Author(s) -
Cao Binrui,
Qiu Penghe,
Mao Chuanbin
Publication year - 2013
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22251
Subject(s) - polyacrylic acid , biocompatibility , iron oxide nanoparticles , transfection , mesenchymal stem cell , gene delivery , chemistry , dispersity , nanoparticle , fluorescence microscope , nanotechnology , stem cell , materials science , biophysics , biochemistry , fluorescence , microbiology and biotechnology , biology , polymer , gene , organic chemistry , physics , quantum mechanics
Novel monodisperse mesoporous iron oxide nanoparticles (m‐IONPs) were synthesized by a postsynthesis etching approach and characterized by electron microscopy. In this approach, solid iron oxide nanoparticles (s‐IONPs) were first prepared following a solvothermal method, and then etched anisotropically by polyacrylic acid to form the mesoporous nanostructures. MTT cytotoxicity assay demonstrated that the m‐IONPs have good biocompatibility with mesenchymal stem cells (MSCs). Owing to their mesoporous structure and good biocompatibility, these monodisperse m‐IONPs were used as a nonviral vector for the delivery of a gene of vascular endothelial growth factor (VEGF) tagged with a green fluorescence protein (GFP) into the hard‐to‐transfect stem cells. Successful gene delivery and transfection were verified by detecting the GFP fluorescence from MSCs using fluorescence microscopy. Our results illustrated that the m‐IONPs synthesized in this work can serve as a potential nonviral carrier in gene therapy where stem cells should be first transfected and then implanted into disease sites for disease treatment. Microsc. Res. Tech. 76:936–941, 2013 . © 2013 Wiley Periodicals, Inc.