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Implementation of a virtual correlative light and transmission electron microscope
Author(s) -
Voigt Tilman,
Zuber Benoît,
Gawatz Gerlinde,
Herrmann Gudrun
Publication year - 2013
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22218
Subject(s) - correlative , electron microscope , conventional transmission electron microscope , electron tomography , transmission (telecommunications) , microscope , transmission electron microscopy , optics , electron , scanning transmission electron microscopy , computer science , materials science , nanotechnology , physics , telecommunications , nuclear physics , philosophy , linguistics
In the long run, the widespread use of slide scanners by pathologists requires an adaptation of teaching methods in histology and cytology in order to target these new possibilities of image processing and presentation via the internet. Accordingly, we were looking for a tool with the possibility to teach microscopic anatomy, histology, and cytology of tissue samples which would be able to combine image data from light and electron microscopes independently of microscope suppliers. With the example of a section through the villus of jejunum, we describe here how to process image data from light and electron microscopes in order to get one image‐stack which allows a correlation of structures from the microscopic anatomic to the cytological level. With commercially available image‐presentation software that we adapted to our needs, we present here a platform which allows for the presentation of this new but also of older material independently of microscope suppliers. Microsc. Res. Tech. 76:679–686, 2013 . © 2013 Wiley Periodicals, Inc.