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Differential expression and regulation of PNA and UEA‐1 bindings in rabbit uterus during preimplantation period
Author(s) -
Guo Bin,
Duan CuiCui,
Wang QuYuan,
Yue ZhanPeng
Publication year - 2013
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22179
Subject(s) - uterus , estrous cycle , agglutinin , andrology , peanut agglutinin , biology , medicine , endocrinology , pregnancy , chemistry , microbiology and biotechnology , lectin , genetics
Peanut agglutinin (PNA) and Ulex europaeus agglutinin‐1 (UEA‐1) were used as probes to study the distribution of β‐gal (1→3) ga1Nac and α‐ l ‐Fucose in rabbit uterus during early pregnancy. PNA binding was mainly localized on the surface of uterine glandular and luminal epithelium. There were no positive signals on day 1 of pregnancy. PNA binding gradually increased from day 2 and reached its highest level on days 3 and 4. The distribution of PNA binding gradually declined from day 5 and reached a low level on day 7. However, UEA‐1 binding was only localized on the luminal epithelial during early pregnancy. A high level of UEA‐1 binding had been found on the luminal epithelium on day 1 of pregnancy and low level of positive signals had been found in the uterus on days 2 and 3. UEA‐1 binding increased gradually and reached its highest level on day 4. Then the distribution of UEA‐1 binding sharply declined and no positive signals were found on days 5–7. The distribution of PNA and UEA‐1 bindings in pseudopregnant uterus was similar to that in normal pregnant uterus. During estrus cycle, there was no detectable PNA binding signal in uterus. But, a high level of UEA‐1 binding was found in the luminal epithelium of estrus uterus. In ovariectomized rabbit uterus, progesterone significantly induced the expression of PNA binding, while estrogen stimulated UEA‐1 binding expression. These results suggested that the distribution of PNA and UEA‐1 bindings in rabbit uterus may be related to rabbit implantation. Microsc. Res. Tech. 76:398–403, 2013 . © 2013 Wiley Periodicals, Inc.