MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in  Escherichia coli | Zendy

Li Xin | Zendy; Hu Rongliu | Zendy; Zhu Wenxue | Zendy; Fan Jinling | Zendy; Pang Xinyue | Zendy; Wang Na | Zendy; Wang Liping | Zendy; Yang Lipeng | Zendy; Zhao Chunyan | Zendy; He Chenyang | Zendy

Premium

MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in Escherichia coli

Author(s) -

Li Xin,

Hu Rongliu,

Zhu Wenxue,

Fan Jinling,

Pang Xinyue,

Wang Na,

Wang Liping,

Yang Lipeng,

Zhao Chunyan,

He Chenyang

Publication year - 2013

Publication title -

microscopy research and technique

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.536

H-Index - 118

eISSN - 1097-0029

pISSN - 1059-910X

DOI - 10.1002/jemt.22152

Subject(s) - hydrogen peroxide , transmission electron microscopy , escherichia coli , staining , chemistry , scanning electron microscope , nuclear chemistry , endogeny , cerium , materials science , inorganic chemistry , biochemistry , biology , nanotechnology , genetics , composite material , gene

The bacterial endogenous hydrogen peroxide (H 2 O 2 ) was detected cytochemically by its reaction with cerium chloride (CeCl 3 ) to produce electron‐dense deposits of cerium perhydroxides. The sequence of fixation and CeCl 3 staining of H 2 O 2 in the processing of transmission electron microscope (TEM) sample preparation is crucial to the localization of endogenous H 2 O 2 in Escherichia coli . In this study, results confirmed that the process that fixation simultaneously with CeCl 3 staining provided optimum effects for H 2 O 2 localization in E. coli . The modified process of TEM provides very efficient protection for H 2 O 2 localization and more accurate quantization for the H 2 O 2 accumulation in bacterial cells. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research