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MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in Escherichia coli
Author(s) -
Li Xin,
Hu Rongliu,
Zhu Wenxue,
Fan Jinling,
Pang Xinyue,
Wang Na,
Wang Liping,
Yang Lipeng,
Zhao Chunyan,
He Chenyang
Publication year - 2013
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22152
Subject(s) - hydrogen peroxide , transmission electron microscopy , escherichia coli , staining , chemistry , scanning electron microscope , nuclear chemistry , endogeny , cerium , materials science , inorganic chemistry , biochemistry , biology , nanotechnology , genetics , composite material , gene
The bacterial endogenous hydrogen peroxide (H 2 O 2 ) was detected cytochemically by its reaction with cerium chloride (CeCl 3 ) to produce electron‐dense deposits of cerium perhydroxides. The sequence of fixation and CeCl 3 staining of H 2 O 2 in the processing of transmission electron microscope (TEM) sample preparation is crucial to the localization of endogenous H 2 O 2 in Escherichia coli . In this study, results confirmed that the process that fixation simultaneously with CeCl 3 staining provided optimum effects for H 2 O 2 localization in E. coli . The modified process of TEM provides very efficient protection for H 2 O 2 localization and more accurate quantization for the H 2 O 2 accumulation in bacterial cells. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.