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Qualitative study of young, adult, and aged wistar rats temporomandibular synovial membrane employing light, scanning, and transmission electron microscopy
Author(s) -
Duro Christiano Cony,
Ciena Adriano Polican,
De Almeida Sonia Regina Yokomizo,
Da Silva Righetti Marta Maria,
De Freitas Grisolia Débora,
Issa João Paulo Mardegan,
Da Silva Marcelo Cavenaghi Pereira,
Watanabe Iisei
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22095
Subject(s) - transmission electron microscopy , scanning electron microscope , membrane , synovial membrane , materials science , pathology , chemistry , anatomy , medicine , biophysics , biology , nanotechnology , immunology , inflammation , composite material , biochemistry
The aim of this study was to analyze the rat temporomandibular joint (TMJ) synovial membrane at different ages using light, scanning, and transmission electron microscopy. Under light microscopic analysis, the TMJ structures were observed such as condyle, capsule, disk, the synovial membrane collagen type, and cells distribution. In the scanning electron microscopy, the synovial membrane surface exhibited a smooth aspect in young animals and there was an increase with ageing in the number of folds. The transmission electron microscopic analysis showed more synoviocytes in the synovial layer in the young group and still a great number of vesicles and cisterns dilation of rough endoplasmic reticulum in the aged group. In the three groups, a dense layer of collagen fibers in the synovial layer and cytoplasmic extensions were clearly seen. It was possible to conclude that synovial membrane structures in aged group showed alterations contributing to the decrease in joint lubrication and in the sliding between disk and joint surfaces. These characteristic will reflect in biomechanics of chewing, and may cause the TMJ disorders, currently observed in clinical processes. Microsc. Res. Tech. © 2012 Wiley Periodicals, Inc.