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Hematopoietic progenitor constituents and adherent cell progenitor morphology isolated from black‐rumped agouti ( Dasyprocta prymnolopha , Wagler 1831) bone marrow
Author(s) -
Da Rocha Andressa Rego,
Alves Flávio Ribeiro,
Neto NapoleãO Martins Argôlo,
Dos Santos Luciano Fonseca,
De Almeida Hatawa Melo,
De Carvalho Yulla Klinger Pereira,
Bezerra Dayseanny De Oliveira,
Ferraz Maíra Soares,
Pessoa Gerson Tavares,
De Carvalho Maria Acelina Martins
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22077
Subject(s) - bone marrow , progenitor cell , trypsinization , biology , stem cell , haematopoiesis , cell , cell culture , microbiology and biotechnology , pathology , anatomy , immunology , medicine , genetics , biochemistry , trypsin , enzyme
Stem cells are present in the adult tissues of most diverse species. Bone marrow is recognized to be the most exploited site to obtain stem cells and cell progenitors. The objective of the present study was to characterize hematopoietic progenitor (HP) morphology and analyze the performance of adherent cell progenitors (ACPs) cultivated in vitro from black‐rumped agouti bone marrow ( Dasyprocta prymnolopha ). Bone marrow aspirates were obtained from tibia crest and used to prepare histological slides and identify cell morphology. Cells were also scattered on culture plates for later isolation, expansion, and quantification. Smears obtained from bone marrow demonstrated HPs at different stages of maturity. In culture, these cells showed fibroblastoid morphology and a strong tendency to form colonies, demonstrated by the presence of cell aggregates, cytoplasmic elongations lying side by side. An 80% cell confluence was observed at 18 days in culture and progressive reduction in the percentage of nonadherent mononuclear cells. After eight passes, a mean cell viability of 96.07% was observed, from a pool of 1.6 × 10 7 cells (ACP). Thirteen 25‐cm 2 culture bottles were trypsinized, resuspended in freezing medium, stored in 14 criotubes at a concentration of 1 × 10 6 cells per milliliter, and placed in liquid nitrogen at −196°C. Agouti bone marrow demonstrated high plasticity, moreover different HP lines, and a population of adherent cells demonstrated morphology similar to mesenchymal stem cells in culture. Microsc. Res. Tech. 2012. © 2012 Wiley Periodicals, Inc.